7UE9
Structure of anti-C3d Fab(3d8b) in complex with C3d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-02-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0. 979257 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.372, 62.229, 85.547 |
Unit cell angles | 90.00, 108.03, 90.00 |
Refinement procedure
Resolution | 49.470 - 1.750 |
R-factor | 0.1725 |
Rwork | 0.171 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ont |
RMSD bond length | 0.013 |
RMSD bond angle | 1.848 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.470 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 73836 | 11680 |
<I/σ(I)> | 15.23 | |
Completeness [%] | 99.5 | |
Redundancy | 3.71 | |
CC(1/2) | 0.998 | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 15% PEG 10,000, 0.1 M bicine pH 7.6 |