7SXH
BIO-8546 bound GSK3beta-axin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-02-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.610, 72.640, 79.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.290 - 2.090 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kpl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.965 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.290 | 2.170 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 23943 | 2242 |
<I/σ(I)> | 14.65 | |
Completeness [%] | 99.3 | |
Redundancy | 6.5 | |
CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M calcium acetate, 0.1M BisTRIS pH 7.0, 5% Glycerol and 17% PEG3350 |