7S0P

Crystal structure of Porcine Factor VIII C2 Domain Bound to Phosphatidylserine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-02-05
Detector	ADSC QUANTUM 1
Wavelength(s)	1.1158
Spacegroup name	I 2 2 2
Unit cell lengths	49.072, 68.323, 105.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.531 - 1.300
R-factor	0.1374
Rwork	0.137
R-free	0.15590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mo3
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.531	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.073	0.764
Rmeas	0.078	0.892
Rpim	0.029	0.440
Number of reflections	42676	1614
<I/σ(I)>	18.3
Completeness [%]	96.5
Redundancy	13.3
CC(1/2)	0.998	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2:1 drop ratio 0.1 M CHES (pH 7.4) 0.1M magnesium acetate 10% ethanol (v/v) Crystal soaked with 5 mM OPLS