7RCO
Crystal structure of human TGF-beta-2 bound to 4A11.V2 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 60.154, 103.479, 196.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.951 - 2.900 |
R-factor | 0.2119 |
Rwork | 0.208 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tfg |
RMSD bond length | 0.013 |
RMSD bond angle | 1.229 |
Data reduction software | AutoProcess |
Data scaling software | Aimless (0.5.28) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12-2829_final) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.060 | 49.060 | 3.080 |
High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
Rmerge | 0.086 | 0.033 | 0.731 |
Rmeas | 0.099 | 0.039 | 0.831 |
Rpim | 0.048 | 0.021 | 0.389 |
Total number of observations | 111744 | 3561 | 18905 |
Number of reflections | 27630 | 1074 | 4429 |
<I/σ(I)> | 11.6 | 29.5 | 1.9 |
Completeness [%] | 98.8 | 93.1 | 99.7 |
Redundancy | 4 | 3.3 | 4.3 |
CC(1/2) | 0.998 | 0.998 | 0.663 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG4000, 0.1 M sodium cacodylate, pH 6.0 |