7R8U
LOX-1 - Structural and Functional Studies of a Receptor Implicated in Atherosclerosis
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.19499 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.908, 89.901, 110.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.492 - 1.901 |
Rwork | 0.186 |
R-free | 0.21810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ypu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.611 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.492 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 57364 | 3538 |
<I/σ(I)> | 1.6 | |
Completeness [%] | 96.9 | |
Redundancy | 6.5 | |
CC(1/2) | 0.995 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M 1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, 1 M Tris base, BICINE, pH 8.5, 50% v/v precipitant mix (25% v/v MPD, 25% PEG1000, 25% w/v PEG3350) |