7PI2
PfCyRPA bound to monoclonal antibody Cy.003 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 354.300, 71.045, 164.789 |
| Unit cell angles | 90.00, 91.16, 90.00 |
Refinement procedure
| Resolution | 88.560 - 3.140 |
| R-factor | 0.2197 |
| Rwork | 0.219 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tih |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 164.760 | 3.210 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 239064 | 15017 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.956 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M NaCl, 0.1 M MES pH 6, 20 % PEG 2000 MME |
