7PGK
HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), in complex with glycosaminoglycan mimic SOS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 60.240, 60.240, 108.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.000 - 2.750 |
R-factor | 0.268 |
Rwork | 0.267 |
R-free | 0.28600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.200 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.113 | |
Rpim | 0.035 | |
Number of reflections | 5609 | 534 |
<I/σ(I)> | 12 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.9 | 10.9 |
CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M imidazole/MES pH 6.5, 0.1 M carboxylic acids (0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M sodium citrate, 0.02 M sodium oxamate and 0.02 M potassium sodium tartrate), 10% w/v PEG 20000 and 20% v/v PEG MME 550 |