7P8L
Crystal structure of Pyrococcus abyssi 8-oxoguanine DNA glycosylase (PabAGOG) in complex with dsDNA containing cytosine opposite to 8-oxoG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-02 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.256, 68.811, 140.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.170 - 1.250 |
R-factor | 0.1364 |
Rwork | 0.135 |
R-free | 0.16100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7oy7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.046 | 0.354 |
Number of reflections | 83399 | 4101 |
<I/σ(I)> | 28.5 | 5.3 |
Completeness [%] | 99.9 | |
Redundancy | 13 | |
CC(1/2) | 0.999 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Na acetate pH 4.5, KCl, MPD |