7P3B
Human RNA ligase RTCB in complex with GMP and Co(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-07 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.906, 96.906, 227.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.990 - 2.300 |
R-factor | 0.1935 |
Rwork | 0.192 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uc2 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.676 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.990 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.068 | 0.648 |
Number of reflections | 48937 | 4769 |
<I/σ(I)> | 11.5 | 1.1 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 26.3 | 25.9 |
CC(1/2) | 0.997 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M sodium acetate pH 5.5, 0.6 M sodium formate, 14% (w/v) PEG 4000, 5 mM cobalt(II) chloride |