7OCM
K1K1H6, a potent recombinant minimal hepatocyte growth factor/scatter factor mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.839, 59.648, 46.289 |
Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
Resolution | 35.890 - 1.700 |
R-factor | 0.1653 |
Rwork | 0.164 |
R-free | 0.19450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bht |
Data reduction software | iMOSFLM |
Data scaling software | XSCALE (VERSION January 10, 2014 BUILT=20140115) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.940 | 44.940 | 1.300 |
High resolution limit [Å] | 1.260 | 4.880 | 1.260 |
Rmerge | 0.069 | 0.063 | 1.424 |
Rmeas | 0.081 | 0.073 | 1.687 |
Rpim | 0.041 | 0.036 | 0.890 |
Total number of observations | 167746 | 3128 | 15200 |
Number of reflections | 45262 | 820 | 4404 |
<I/σ(I)> | 8.4 | 28.3 | 0.9 |
Completeness [%] | 99.8 | 99.5 | 99.2 |
Redundancy | 3.7 | 3.8 | 3.5 |
CC(1/2) | 0.998 | 0.993 | 0.392 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290.15 | 100 mM MOPS/HEPES pH 7.5, 30 mM sodium nitrate, 30 mM sodium phosphate dibasic, 30 mM ammonium sulphate, 20% v/v glycerol, 10% w/v PEG4000 |