7MNZ
Crystal Structure of Nup358/RanBP2 Ran-binding domain 4 in complex with Ran-GPPNHP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 31 |
Unit cell lengths | 142.690, 142.690, 96.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.360 - 2.350 |
R-factor | 0.1854 |
Rwork | 0.183 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rrp |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.360 | 29.360 | 2.430 |
High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
Rmeas | 0.154 | 0.061 | 1.835 |
Rpim | 0.048 | 0.019 | 0.591 |
Total number of observations | 935004 | 90644 | 86810 |
Number of reflections | 91578 | 9162 | 9170 |
<I/σ(I)> | 12.4 | 30.3 | 1.8 |
Completeness [%] | 99.9 | 99.9 | 99.8 |
Redundancy | 10.2 | 9.9 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 20% w/v PEG3350, 0.1 M ammonium sulfate, 0.1 M HEPES |