7KGZ
FMN-binding beta-glucuronidase from Roseburia hominis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0330 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.354, 161.142, 86.947 |
Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
Resolution | 39.070 - 2.400 |
R-factor | 0.1428 |
Rwork | 0.141 |
R-free | 0.19520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mvh |
RMSD bond length | 0.007 |
RMSD bond angle | 0.878 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.070 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.050 | 0.124 |
Rmeas | 0.060 | 0.147 |
Rpim | 0.032 | 0.078 |
Number of reflections | 55112 | 5470 |
<I/σ(I)> | 18.68 | 8.37 |
Completeness [%] | 98.9 | 98.38 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.997 | 0.981 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12.5 mg/mL protein, 0.2 M lithium chloride, 20% (w/v) PEG 3350 |