7EAX

Crystal complex of p53-V272M and antimony ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978520
Spacegroup name	P 1 21 1
Unit cell lengths	68.876, 72.551, 85.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.530 - 2.550
R-factor	0.2076
Rwork	0.206
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ocj
RMSD bond length	0.004
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.530	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.101	0.546
Rmeas	0.141	0.743
Rpim	0.075	0.389
Number of reflections	26974	3329
<I/σ(I)>	8.6	2.1
Completeness [%]	98.1	99.4
Redundancy	3.4	3.6
CC(1/2)	0.992	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris pH 8.5, 10% w/v PEG3350 and 0.2M Magnesium chloride