7D35
Human LC8 bound to ebola virus VP35(67-76)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2019-12-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 43.426, 43.426, 205.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.330 - 2.401 |
R-factor | 0.234 |
Rwork | 0.228 |
R-free | 0.29170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cmi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.911 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.094 | 0.310 |
Number of reflections | 4869 | 225 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 95.0 | |
Redundancy | 4.7 | |
CC(1/2) | 0.975 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200mM ammonium citrate dibasic, 200mM ammonium citrate tribasic (pH 5.5), 30% (w/v) polyethylene glycol 3350 |