6YSQ
The hC4Nb8 complement inhibitory nanobody in complex with C4b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.200, 89.510, 231.200 |
Unit cell angles | 90.00, 97.55, 90.00 |
Refinement procedure
Resolution | 48.260 - 3.300 |
R-factor | 0.2216 |
Rwork | 0.221 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jtw |
RMSD bond length | 0.005 |
RMSD bond angle | 0.929 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.260 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 80517 | 7914 |
<I/σ(I)> | 11.73 | 0.65 |
Completeness [%] | 99.9 | 99.94 |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.998 | 0.341 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292.15 | The reservoir containing 100 mM HEPES-NaOH pH=7, 10% w/w PEG4000, 10% v/v 2-propanol was mixed 1:1 with the concentrated protein sample. |