6X3K

Hsa Siglec and Unique domains in complex with 6S-sialy-Lewisx

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.635, 58.173, 75.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.2366
Rwork	0.236
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6efc
RMSD bond length	0.027
RMSD bond angle	2.060
Phasing software	PHENIX (1.18_3855)
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rpim		0.318
Number of reflections	7619	363
<I/σ(I)>	15.6
Completeness [%]	98.7
Redundancy	4.9
CC(1/2)	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	21.6 mg/ml protein in 20 mM Tris-HCl, pH 7.2, Mix 1uL protein solution with 2uL reservoir solution. Reservoir solution: 0.1 M Succinate/Phosphate/Glycine pH 10.0 and 25% PEG 3350