6W3Q
APE1 exonuclease substrate complex L104R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-05-31 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.050, 66.604, 90.676 |
Unit cell angles | 90.00, 109.41, 90.00 |
Refinement procedure
Resolution | 47.570 - 2.490 |
R-factor | 0.2235 |
Rwork | 0.222 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wn4 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.520 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.570 | 2.590 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmeas | 0.096 | 3.039 |
Number of reflections | 28101 | 3218 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 96.2 | |
Redundancy | 3.5 | |
CC(1/2) | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 7% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride |