6W3N
APE1 exonuclease substrate complex D148E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-07 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.554, 65.074, 91.424 |
Unit cell angles | 90.00, 109.93, 90.00 |
Refinement procedure
Resolution | 24.760 - 2.690 |
R-factor | 0.2549 |
Rwork | 0.249 |
R-free | 0.30840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w4n |
RMSD bond length | 0.012 |
RMSD bond angle | 1.359 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.750 |
High resolution limit [Å] | 2.690 | 2.700 |
Rmeas | 0.210 | 1.000 |
Number of reflections | 37101 | 1645 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 96.2 | |
Redundancy | 2.9 | |
CC(1/2) | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 7% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride |