6VNB
JAK2 JH1 in complex with BL2-084
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 |
Unit cell lengths | 113.053, 113.053, 70.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.156 - 2.190 |
R-factor | 0.2172 |
Rwork | 0.216 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xa4 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.200 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmeas | 0.206 | |
Number of reflections | 89833 | 4598 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.990 | 0.160 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M Bis-Tris pH 5.5, 0.2 M NaCl, 25% PEG 3350 |