6TVS
Crystal structure of the haemagglutinin mutant (Gln226Leu) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.340, 218.360, 73.060 |
Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
Resolution | 51.540 - 1.900 |
R-factor | 0.2308 |
Rwork | 0.230 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d00 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.078 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.280 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 164307 | 8151 |
<I/σ(I)> | 11.3 | 1.3 |
Completeness [%] | 99.6 | |
Redundancy | 3.1 | |
CC(1/2) | 0.998 | 0.487 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 4% PEG3350, 0.1M HEPES pH 7.5 |