6TQT

The crystal structure of the MSP domain of human MOSPD2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-26
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	28.114, 51.216, 78.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.230 - 1.500
R-factor	0.136
Rwork	0.134
R-free	0.18260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tqs
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (dev-3699)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.800	78.800	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.129	0.086	0.364
Rmeas	0.137	0.093	0.484
Rpim	0.046	0.032	0.316
Total number of observations	124110	1150	1329
Number of reflections	18679	155	720
<I/σ(I)>	9.8	13.8	1.8
Completeness [%]	98.5	99.5	80.5
Redundancy	6.6	7.4	1.8
CC(1/2)	0.995	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	40% PEG 300, 0.1 M Sodium Phosphate Citrate pH 4.2