6SPR
Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298 complexed with beta-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.310, 45.110, 86.210 |
Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
Resolution | 48.560 - 1.480 |
R-factor | 0.1329 |
Rwork | 0.131 |
R-free | 0.16780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qqt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 1.570 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 96417 | 15234 |
<I/σ(I)> | 9.2 | 1.3 |
Completeness [%] | 99.6 | |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0 |