6SB1
Crystal structure of murine perforin-2 P2 domain crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-23 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.96861 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.530, 31.270, 103.650 |
Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
Resolution | 57.632 - 2.050 |
R-factor | 0.2135 |
Rwork | 0.212 |
R-free | 0.24280 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.093 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2645) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.980 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 28963 | 7980 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M MES pH6.0, 10% PEG6000, 4mM L-cysteine |