6RPJ
A Non-blocking anti-CTLA-4 Nanobody complexed with CTLA-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-11 |
| Detector | DECTRIS PILATUS3 R 300K |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 309.977, 75.708, 83.047 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 47.570 - 3.250 |
| R-factor | 0.2495 |
| Rwork | 0.249 |
| R-free | 0.26155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.146 | 0.718 |
| Number of reflections | 30159 | 1926 |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG400 |
