6Q42
Crystal Structure of Human Pancreatic Phospholipase A2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.610, 58.951, 122.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.000 - 1.900 |
Rwork | 0.175 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b00 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.200 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.119 | 0.975 |
Number of reflections | 18069 | |
<I/σ(I)> | 8.8 | 1.9 |
Completeness [%] | 99.3 | 98 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 32% (w/v) PEG4K, 0.8M Lithium chloride, Tris-HCl |