6LB4
Crystal structure of dimeric RXR-LBD complexed with NEt-3ME and TIF2 co-activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.298, 66.298, 109.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.340 - 1.500 |
R-factor | 0.1752 |
Rwork | 0.174 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k6i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.044 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 39809 | 6329 |
<I/σ(I)> | 15.1 | |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 11.8 | |
CC(1/2) | 0.999 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium Acetate 18 % PEG3350 0.1 M Spermine tetrahydrochroride |