6KIE
Crystal structure of human leucyl-tRNA synthetase, Leu-AMS-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2017-06-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 138.774, 138.774, 448.843 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.848 - 3.150 |
R-factor | 0.23 |
Rwork | 0.229 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wz2 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.110 | 0.056 | 0.423 |
Rmeas | 0.119 | 0.060 | 0.495 |
Rpim | 0.043 | 0.020 | 0.250 |
Total number of observations | 280543 | ||
Number of reflections | 46132 | 2472 | 2171 |
<I/σ(I)> | 10.4 | ||
Completeness [%] | 96.4 | 90.9 | 93 |
Redundancy | 6.1 | 7.9 | 3.3 |
CC(1/2) | 0.996 | 0.078 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24% PEG3350 |