6HYO

Structure of ULK1 LIR motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9159
Spacegroup name	I 2 3
Unit cell lengths	100.720, 100.720, 100.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.119 - 1.070
R-factor	0.1425
Rwork	0.142
R-free	0.16080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.013
RMSD bond angle	1.392
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.120	1.100
High resolution limit [Å]	1.070	1.070
Rmerge	0.040	1.650
Rmeas	0.050	1.710
Rpim	0.010	0.440
Number of reflections	74520	7428
<I/σ(I)>	23.9	1.6
Completeness [%]	99.9	100
Redundancy	18.6	14.6
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M MgCl2. 0.1 M Na cacodylate pH 6.5, 20% PEG1000