6FNW
Structure of a volume-regulated anion channel of the LRRC8 family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-11-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1, 2.05 |
Spacegroup name | P 65 |
Unit cell lengths | 140.130, 140.130, 42.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.452 - 1.800 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.20820 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.270 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.452 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 1.580 | |
Number of reflections | 44375 | 6557 |
<I/σ(I)> | 27.05 | 2.27 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 20.2 | 19.8 |
CC(1/2) | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 22% PEG 3350, 0.2M Na/K tartrate, 0.1M Bis-Tris propane pH 8.5 |