6E7K
Structure of the lipoprotein lipase GPIHBP1 complex that mediates plasma triglyceride hydrolysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 125 |
Detector technology | PIXEL |
Collection date | 2017-12-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 101.948, 153.206, 95.783 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.410 - 2.800 |
R-factor | 0.19738 |
Rwork | 0.195 |
R-free | 0.23545 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hpl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.433 |
Data reduction software | HKL-2000 (v716.1) |
Data scaling software | HKL-2000 (v716.1) |
Phasing software | PHASER (v2.7.17) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.800 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 37662 | |
<I/σ(I)> | 13.5 | 1.5 |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.985 | 0.728 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM magnesium acetate, 20% PEG3350 |