6DUK
EGFR with an allosteric inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2015-11-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 57.481, 94.711, 97.398 |
Unit cell angles | 70.38, 77.46, 79.14 |
Refinement procedure
Resolution | 88.504 - 2.200 |
R-factor | 0.1906 |
Rwork | 0.190 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d41 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.164 |
Data reduction software | ABS |
Data scaling software | ABS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.460 | 2.120 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.106 | 1.281 |
Rpim | 0.063 | 0.912 |
Number of reflections | 108372 | 13035 |
<I/σ(I)> | 25.75 | |
Completeness [%] | 97.2 | |
Redundancy | 12.4 | |
CC(1/2) | 0.996 | 0.474 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, 22% PEG3350, 150 mM NaCl |