6ALE
A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-07-12 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.653, 66.913, 64.712 |
Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
Resolution | 24.193 - 2.500 |
R-factor | 0.1912 |
Rwork | 0.188 |
R-free | 0.25510 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.127 |
Data reduction software | XPREP |
Data scaling software | XPREP |
Phasing software | PHASES |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.720 |
High resolution limit [Å] | 2.500 |
Number of reflections | 11420 |
<I/σ(I)> | 13.3 |
Completeness [%] | 100.0 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.1 M Sodium Citrate Tribasic dehydrate 0.6 M Ammonium sulfate 1.4 M Lithium sulfate monohydrate |