6AEB
Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 361.853, 71.130, 198.320 |
Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
Resolution | 49.452 - 3.004 |
R-factor | 0.2321 |
Rwork | 0.231 |
R-free | 0.28280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oo8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.119 | |
Number of reflections | 98977 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 99.3 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Hepes, PH 7.5, 0.2M Ammonium sulfate, 10% ispropanol, and 16% (w/v) PEG4000 |