5ZIC
Crystal structure of human GnT-V luminal domain in complex with acceptor sugar
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-11-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.421, 89.202, 92.234 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 44.601 - 2.100 |
| R-factor | 0.2173 |
| Rwork | 0.215 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zib |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.664 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.601 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.038 | 0.494 |
| Number of reflections | 63177 | |
| <I/σ(I)> | 16.9 | 1.7 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Hepes (pH 7.0), 0.1M magnesium chloride, 15 % (w/v) PEG 4000 |
