5XZV
Crystal structure of Rad53 1-466 in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 116.690, 116.690, 140.804 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.880 - 3.100 |
R-factor | 0.273 |
Rwork | 0.272 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.668 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.064 | 0.848 |
Number of reflections | 20517 | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 11 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4 |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4 |