5VQP
Crystal structure of human pro-TGF-beta1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03318 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 104.390, 104.390, 141.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.477 - 2.900 |
R-factor | 0.2541 |
Rwork | 0.252 |
R-free | 0.29290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rjr |
RMSD bond length | 0.004 |
RMSD bond angle | 0.641 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.170 | 6.000 |
Number of reflections | 10672 | 753 |
<I/σ(I)> | 12.32 | 0.48 |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.5 | |
CC(1/2) | 0.999 | 0.150 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 289 | 100 mM MES pH6.3, 1.68 M Ammonium Sulfate, 11% Dioxane |