5TQ0

Crystal structure of amino terminal domains of the NMDA receptor subunit GluN1 and GluN2A in the presence of EDTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.28
Spacegroup name	P 21 21 21
Unit cell lengths	79.980, 118.346, 156.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.569 - 2.700
R-factor	0.2122
Rwork	0.208
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpw
RMSD bond length	0.004
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Rmerge	0.129
Number of reflections	77929
<I/σ(I)>	7.72
Completeness [%]	99.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.8 M Ammonium sulfate, 2.5% Isopropanol