5OX5
HIF prolyl hydroxylase 2 (PHD2/ EGLN1) in complex with CCT6, a GSK1278863-related compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9800 |
Spacegroup name | P 63 |
Unit cell lengths | 110.147, 110.147, 39.368 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.690 - 2.251 |
R-factor | 0.1761 |
Rwork | 0.175 |
R-free | 0.20480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bqx |
RMSD bond length | 0.003 |
RMSD bond angle | 0.553 |
Data reduction software | HKL-2000 (706E) |
Data scaling software | HKL-2000 (706E) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.690 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.138 | 0.953 |
Rpim | 0.064 | 0.449 |
Number of reflections | 13170 | 1314 |
<I/σ(I)> | 12.9 | 2.1 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.7 | 5.7 |
CC(1/2) | 0.994 | 0.598 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.5 M sodium citrate tribasic dehydrate pH 6.5, Sitting drop (300 nl), protein-to-well ratio, 1:1. |