5LSO
Crystal structure of SPF45 UHM domain with cyclic peptide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.600, 73.590, 45.010 |
Unit cell angles | 90.00, 97.90, 90.00 |
Refinement procedure
Resolution | 38.130 - 2.220 |
R-factor | 0.1972 |
Rwork | 0.181 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2peh |
RMSD bond length | 0.014 |
RMSD bond angle | 1.759 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.130 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.076 | 0.212 |
Number of reflections | 11806 | |
<I/σ(I)> | 14.61 | 5.29 |
Completeness [%] | 98.0 | 88 |
Redundancy | 5.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM MES pH 6, 70% MPD |