5LS6
Structure of Human Polycomb Repressive Complex 2 (PRC2) with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.310, 170.270, 275.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.840 - 3.470 |
| R-factor | 0.23215 |
| Rwork | 0.229 |
| R-free | 0.29866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hyn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.840 | 3.560 |
| High resolution limit [Å] | 3.470 | 3.470 |
| Rmerge | 0.300 | 1.810 |
| Number of reflections | 76561 | |
| <I/σ(I)> | 6.9 | 1.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.67 | 291 | 24% PEG3350, 400mM Ammonian Citrate pH6.67 |
