5LHB
POLYADPRIBOSYL GLYCOSIDASE IN COMPLEX WITH PDD00017262
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.010, 89.730, 95.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.610 - 2.230 |
| R-factor | 0.1647 |
| Rwork | 0.162 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0d |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.510 | 2.370 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.109 | 0.614 |
| Number of reflections | 28669 | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 36.4MM PCTP PH 4, 63.6MM PCTP PH 10, 0.2M AMMONIUM SULPHATE |
