5LGS
Crystal structure of mouse CARM1 in complex with ligand P2C3u
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.986, 98.596, 208.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.971 - 2.100 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ih3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.516 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.971 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.132 | 2.753 |
Number of reflections | 90877 | |
<I/σ(I)> | 10.4 | 0.7 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.7 | 6.9 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.5 100 mM PEG 3350 20 % A.S. 200 mM |