5JEM

Complex of IRF-3 with CBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2015-10-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 1 21 1
Unit cell lengths	85.230, 88.660, 92.810
Unit cell angles	90.00, 113.09, 90.00

Refinement procedure

Resolution	36.992 - 2.500
R-factor	0.1943
Rwork	0.192
R-free	0.24200
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.992
High resolution limit [Å]	2.500
Number of reflections	42426
<I/σ(I)>	6.2
Completeness [%]	96.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES pH 7.5, 25% PEG 3350