5JEI
Crystal structure of the GluA2 LBD in complex with FW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.465, 44.425, 47.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.374 - 1.229 |
R-factor | 0.122 |
Rwork | 0.121 |
R-free | 0.14730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.492 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.374 | 1.300 |
High resolution limit [Å] | 1.229 | 1.229 |
Rmerge | 0.700 | |
Rpim | 0.069 | |
Number of reflections | 75826 | |
<I/σ(I)> | 14.52 | 2.26 |
Completeness [%] | 96.7 | 92.8 |
Redundancy | 4.07 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 50.0 % v/v PEG 200, 200 mM Sodium Chloride, 100 mM Sodium/Potassium Phosphate pH 6.2 |