5IZU
A new binding site outside the canonical PDZ domain determines the specific interaction between Shank and SAPAP and their function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 72.824, 72.824, 116.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.763 - 2.494 |
| R-factor | 0.1863 |
| Rwork | 0.183 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q3o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.494 |
| Number of reflections | 12561 |
| <I/σ(I)> | 35.2 |
| Completeness [%] | 99.9 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 289 | 0.1 M Bis-Tris, 3.0 M NaCl |
