5HP2
Human Adenosine Deaminase Acting on dsRNA (ADAR2) bound to dsRNA sequence derived from S. cerevisiae BDF2 gene with AU basepair at reaction site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.320, 106.680, 120.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.526 - 2.983 |
| R-factor | 0.1708 |
| Rwork | 0.167 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zy7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.400 | 3.060 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.144 | 0.863 |
| Number of reflections | 21442 | |
| <I/σ(I)> | 10 | 1.88 |
| Completeness [%] | 97.3 | 90 |
| Redundancy | 4.787 | 4.562 |
| CC(1/2) | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG10000 |
