5H1M
Crystal structure of WD40 repeat domains of Gemin5 in complex with M7G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.534, 120.907, 61.257 |
| Unit cell angles | 90.00, 117.42, 90.00 |
Refinement procedure
| Resolution | 29.341 - 2.492 |
| R-factor | 0.1849 |
| Rwork | 0.182 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h1j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.070 | 0.040 | 0.609 |
| Rmeas | 0.084 | 0.047 | 0.723 |
| Rpim | 0.045 | 0.026 | 0.387 |
| Total number of observations | 89686 | ||
| Number of reflections | 26965 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 99.1 | 96.7 | 99.6 |
| Redundancy | 3.3 | 3.4 | 3.3 |
| CC(1/2) | 0.996 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES (pH 7.5), 14% PEG 8000, 25mM MgCl2 |
