5FON
Crystal structure of the Cryptosporidium muris cytosolic leucyl-tRNA synthetase editing domain (apo structure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.708, 107.708, 311.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.700 |
| R-factor | 0.20563 |
| Rwork | 0.203 |
| R-free | 0.25561 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfg |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.841 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.930 |
| Number of reflections | 51501 | |
| <I/σ(I)> | 30 | 2.7 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 8.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.1 M MES (PH 6.8), 2% ETHANOL AND 10% (W/V) PEG 20000. 20% (V/V) ETHYLENE GLYCOL WAS USED AS CRYOPROTECTANT. |
