5EUL

Structure of the SecA-SecY complex with a translocating polypeptide substrate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.27820
Spacegroup name	P 61 2 2
Unit cell lengths	127.798, 127.798, 554.772
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.852 - 3.700
R-factor	0.2962
Rwork	0.295
R-free	0.31480
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	3.800
High resolution limit [Å]	3.700	3.700
Rmerge	0.085
Number of reflections	53847
<I/σ(I)>	9.3	0.4
Completeness [%]	99.0	100
Redundancy	10.6	10.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	21-24% polyethylene glycol 1500, 100mM Tris-HCl pH8.5, 50-100 mM MgAc2, 2% 2-methyl-2,4-pentandiol