5ECG
Crystal structure of the BRCT domains of 53BP1 in complex with p53 and H2AX-pSer139 (gammaH2AX)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9173 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.264, 94.498, 131.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.056 - 3.000 |
R-factor | 0.2067 |
Rwork | 0.203 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kzy |
RMSD bond length | 0.004 |
RMSD bond angle | 0.545 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.060 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 18153 | |
<I/σ(I)> | 11.6 | 1.6 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200mM NaF, 100mM Bis-Tris Propane pH 6.5, 20% (w/v) PEG 3,350 |